IPATIMUP - Institute of Molecular Pathology and Immunology of University of Porto, Porto, Portugal.
Histopathology. 2012 May;60(6):992-8. doi: 10.1111/j.1365-2559.2012.04190.x. Epub 2012 Feb 20.
To characterize the variation in virulence of Helicobacter pylori associated with CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs, and to explore its relationship with the histopathological features of chronic gastritis and with the development of gastric carcinoma.
A total of 169 H. pylori-infected patients with chronic gastritis and gastric carcinoma were studied. The presence of cagA and the number and type of EPIYA motifs were determined by polymerase chain reaction. Infection with strains harbouring two or more CagA EPIYA C motifs was associated with the presence of surface epithelial damage, and with atrophic gastritis and gastric carcinoma. The magnitude of risk for atrophic gastritis and gastric carcinoma increased with increasing number of EPIYA C motifs: strains with one EPIYA C motif conferred a risk (odds ratio [OR]) of 7.3 [95% confidence interval (CI) 2.1-25] for atrophic gastritis, whereas strains with two or more EPIYA C motifs conferred a risk (OR) of 12 (95% CI 2.5-58); strains with one EPIYA C motif conferred a risk (OR) of 17 (95% CI 5.4-55) for gastric carcinoma, whereas strains with two or more EPIYA C motifs conferred a risk (OR) of 51 (95% CI 13-198).
Characterization of the number of H. pylori EPIYA C motifs is important in better defining gastric carcinoma risk.
描述与 CagA Glu-Pro-Ile-Tyr-Ala（EPIYA）基序相关的幽门螺杆菌毒力的变化，并探讨其与慢性萎缩性胃炎的组织病理学特征以及胃癌发生的关系。
共研究了 169 例慢性萎缩性胃炎和胃癌的 H. pylori 感染患者。通过聚合酶链反应确定 cagA 的存在以及 EPIYA 基序的数量和类型。携带两个或更多 CagA EPIYA C 基序的菌株与表面上皮损伤以及萎缩性胃炎和胃癌有关。随着 EPIYA C 基序数量的增加，萎缩性胃炎和胃癌的风险增加：携带一个 EPIYA C 基序的菌株发生萎缩性胃炎的风险（比值比[OR]）为 7.3（95%置信区间[CI] 2.1-25），而携带两个或更多 EPIYA C 基序的菌株发生萎缩性胃炎的风险（OR）为 12（95%CI 2.5-58）；携带一个 EPIYA C 基序的菌株发生胃癌的风险（OR）为 17（95%CI 5.4-55），而携带两个或更多 EPIYA C 基序的菌株发生胃癌的风险（OR）为 51（95%CI 13-198）。
确定 H. pylori EPIYA C 基序的数量对于更好地定义胃癌风险很重要。
