Departments of Pathology, Osaka University Graduate School of Medicine, Yamada-oka, Suita, Japan.
Lab Invest. 2012 Apr;92(4):606-14. doi: 10.1038/labinvest.2012.4. Epub 2012 Feb 20.
Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics.
具有致瘤潜能的肿瘤细胞可能局限于一小部分细胞，称为癌症起始细胞（CICs）。CICs 在体外有效地形成集落，产生 CIC 和非 CIC 群体，维持低水平的活性氧（ROS），表现出高醛脱氢酶（ALDH）活性，并且大多数处于细胞周期的静止状态。霍奇金淋巴瘤（HL）的 CIC 体积小，ROS 水平低。在 HL 细胞系中检查了 ROS 水平和 CIC 中 ALDH 活性之间的关系。ROS 低和 ALDH 高群体在半固体培养中比 ROS 高和 ALDH 低群体更有效地形成集落。ALDH 高群体产生 ALDH 低和高群体，而 ALDH 低群体很少产生 ALDH 高群体。ROS 低细胞的静止状态细胞数量明显多于 ROS 高细胞，而 ALDH 高和 ALDH 低细胞的数量彼此相当。这些发现表明，ALDH 高和 ROS 低细胞具有类似 CIC 的潜能，但它们在细胞周期状态上存在差异，表明 CIC 由具有异质特征的细胞组成。
