Reimer G, Maibach M, Leonhardi G
Arch Dermatol Res. 1979 May 4;264(3):265-73. doi: 10.1007/BF00412653.
Sera of patients with various malignancies are known to contain DNA-binding proteins (DBP) which are not present in sera of normal individuals. In this paper sera of patients with malignant melanoma (MM) were examined as to whether characteristic DBP are present, too. DBP are isolated by DNA-affinity chromatography and represent 0.5-0.9% of all serum proteins. After separation of the DBP by SDS slab gel electrophoresis no typical DBP is detectable in sera of MM-patients. However, quantitative differences are found in sera of patients in the clinical stages I-III and/or tumor level 3-5: 1. All 9 sera of patients who had clinical signs of MM contain more DBP with molecular weight (mw) of 20,000-24,000 dalton than control sera. However, these DBP are only increased in 30% of the 22 sera from MM-patients who had clinical signs for 13-73 months after tumor excision. 2. All sera of the 10 MM-patients of whom sera were drawn twice after tumor excision at an interval of 7-46 months without clinical signs, showed a reduction of DBP with mw 30,000, 68,000, and 165,000.
已知各种恶性肿瘤患者的血清中含有正常个体血清中不存在的DNA结合蛋白（DBP）。在本文中，对恶性黑色素瘤（MM）患者的血清进行了检测，以确定是否也存在特征性的DBP。通过DNA亲和层析分离出DBP，其占所有血清蛋白的0.5 - 0.9%。在通过SDS平板凝胶电泳分离DBP后，在MM患者的血清中未检测到典型的DBP。然而，在临床I - III期和/或肿瘤水平为3 - 5级的患者血清中发现了定量差异：1. 所有9例有MM临床症状的患者血清中，分子量（mw）为20,000 - 24,000道尔顿的DBP比对照血清中的更多。然而，在肿瘤切除后有13 - 73个月临床症状的22例MM患者血清中，这些DBP仅在30%的血清中有所增加。2. 10例MM患者在肿瘤切除后间隔7 - 46个月且无临床症状时两次采血的所有血清中，分子量为30,000、68,000和165,000的DBP均减少。
