Kubinski H, Javid M
Science. 1973 Oct 19;182(4109):296-7. doi: 10.1126/science.182.4109.296.
Cerebrospinal fluid (CSF) contains two groups of proteins that bind tightly to DNA and to polyriboguanylic acid, respectively. In certain diseases the amounts of a given nucleic acid bound by a constant volume of CSF may increase, while in others the amount of such proteins may be reduced. Binding of polyriboguanylic acid increased in CSF samples from patients with brain tumors, stroke, multiple sclerosis, and communicating hydrocephalus, but it significantly decreased in CSF samples from patients with obstructive hydrocephalus. These increases may or may not be proportional to the rise in total CSF proteins characteristic for these diseases. Elevated binding of DNA was observed in samples from patients with hydrocephalus, epilepsy, and cortical atrophy. The technique described may be applicable to the diagnosis of a variety of diseases of the central nervous system.
脑脊液（CSF）含有两组分别与DNA和聚核糖鸟苷酸紧密结合的蛋白质。在某些疾病中，一定体积的脑脊液所结合的特定核酸量可能会增加，而在其他疾病中，此类蛋白质的量可能会减少。脑肿瘤、中风、多发性硬化症和交通性脑积水患者的脑脊液样本中，聚核糖鸟苷酸的结合增加，但在梗阻性脑积水患者的脑脊液样本中则显著减少。这些增加可能与这些疾病所特有的脑脊液总蛋白升高成正比，也可能不成正比。脑积水、癫痫和皮质萎缩患者的样本中观察到DNA结合升高。所描述的技术可能适用于多种中枢神经系统疾病的诊断。
