Hudson I R, Cavill I A, Cooke A, Holland B M, Hoy T G, Trevett D, Turner T L, Wardrop C A
Queen Mother's Hospital, Glasgow, United Kingdom.
Pediatr Res. 1990 Sep;28(3):199-202. doi: 10.1203/00006450-199009000-00006.
Determination of circulating red cell volume (RCV) in anemic preterm infants is, in theory, a better indicator of transfusion needs than Hb concentration. Our study reports the results of RCV measurement using biotin labeling of red cells on 40 occasions in preterm infants of 25-34 wk gestation. In 20 infants, who had estimations made within 24 h of birth, the RCV varied between 17.7 and 66 mL/kg. Twenty measurements were made at a later age at the time of a blood transfusion. RCV values were between 13.1 and 41.5 mL/kg before transfusion. In 13 infants, RCV was determined simultaneously using two methods, biotin and dilution of autologous HbF with donor HbA at transfusion. There was no significant difference between the results of RCV estimations using these two methods. Our study demonstrates that biotin labeling is an effective method for determining RCV in preterm infants.
理论上，测定贫血早产儿的循环红细胞容量（RCV）比血红蛋白浓度更能准确反映输血需求。我们的研究报告了对25至34周胎龄的早产儿进行40次红细胞生物素标记法测量RCV的结果。在出生后24小时内进行测量的20名婴儿中，RCV在17.7至66 mL/kg之间。另外20次测量是在输血时较晚阶段进行的。输血前RCV值在13.1至41.5 mL/kg之间。在13名婴儿中，同时使用生物素法和输血时用供体血红蛋白A稀释自体血红蛋白F这两种方法测定RCV。这两种方法测得的RCV结果无显著差异。我们的研究表明，生物素标记法是测定早产儿RCV的有效方法。
