Mycology and Plant Pathology Group, Plant Science Division, Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, India.
Folia Microbiol (Praha). 2012 Mar;57(2):107-14. doi: 10.1007/s12223-012-0100-2. Epub 2012 Feb 22.
In the present report, nutritional requirement for the culture of two lichen species Ramalina nervulosa and Ramalina pacifica were studied in order to enhance their growth rate and antioxidant metabolite production. Extract of R. nervulosa cultured in Bold's basal medium (BBM) showed higher antioxidant activity than R. pacifica cultured in Murashige and Skoog (MS) medium. The lichen species were sub-cultured in standardized nutrient media. R. nervulosa in BBM (1% glucose, 50 ppb asparagines, pH 6.5) yielded 2.76 g biomass with 26.18 mg sekikaic acid, 24.32 mg usnic acid/g dry biomass in a period of 60 days. R. pacifica in MS media (3% sucrose, 100 ppb thiamine, pH 5.9) yielded 3.54 g biomass and 58.92 mg salazinic acid, 40.16 mg usnic acid in the same time period. The standardized culture conditions implemented on bioreactor, R. nervulosa yielded 17.7 g biomass with the production of sekikaic acid 122.8 mg, usnic acid 75.4 mg in 4.5 days. R. pacifica produced 10.3 g biomass along with salazinic acid 200 mg and usnic acid 136.8 mg in the same duration. Lichen secondary metabolites produced in bioreactor showed moderate antioxidant activity; sekikaic acid 42% to 56.4%; salazinic acid 33.6% to 41.9% and usnic acid 19.9% to 29.5%.
在本报告中，为了提高两种地衣物种（Ramalina nervulosa 和 Ramalina pacifica）的生长速度和抗氧化代谢产物的产量，研究了它们的营养需求。在 Bold's 基础培养基（BBM）中培养的 Ramalina nervulosa 提取物显示出比在 Murashige 和 Skoog（MS）培养基中培养的 Ramalina pacifica 更高的抗氧化活性。将地衣物种在标准化营养培养基中进行传代培养。在 BBM（1%葡萄糖、50ppb 天冬酰胺、pH6.5）中培养的 Ramalina nervulosa 在 60 天内产生了 2.76g 生物量，其中 26.18mg 雪口酸、24.32mg 地衣酸/g 干生物量。在 MS 培养基（3%蔗糖、100ppb 硫胺素、pH5.9）中培养的 Ramalina pacifica 在同一时间内产生了 3.54g 生物量和 58.92mg 雪茶酸、40.16mg 地衣酸。在生物反应器上实施的标准化培养条件下，Ramalina nervulosa 在 4.5 天内产生了 17.7g 生物量，雪口酸产量为 122.8mg，地衣酸产量为 75.4mg。在同一时间内，Ramalina pacifica 产生了 10.3g 生物量，同时产生了 200mg 雪茶酸和 136.8mg 地衣酸。生物反应器中产生的地衣次生代谢产物具有中等的抗氧化活性；雪口酸 42%至 56.4%；雪茶酸 33.6%至 41.9%；地衣酸 19.9%至 29.5%。
