Verma Neeraj, Behera B C, Makhija Urmila
Plant Science Division, Agharkar Research Institute, Pune, MS, India.
Appl Biochem Biotechnol. 2008 Dec;151(2-3):167-81. doi: 10.1007/s12010-008-8164-9. Epub 2008 Feb 26.
Antioxidative and hepatoprotective activity of a cultured lichen Usnea ghattensis has been studied. The methanolic extract of cultured lichen U. ghattensis showed good antioxidant activity by preventing lipid peroxidation by 67% and 86% in Trolox-equivalent antioxidant capacity at 20 microg/ml. It also showed superoxide, 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, and hydroxyl radical-scavenging activity, 89%, 89.6%, 94.8%, and 89.6%, respectively, and found levels higher then that known for the synthetic antioxidants butylated hydroxytoluene, butylated hydroxyanisol, and quercetin at 20 microg/ml concentration. The cultured lichen extract also showed hepatoprotection against ethanol-induced toxicity in the mice liver slice culture model by a significant decrease in the antioxidant enzymes, glutathione peroxidase, catalase, and superoxide dismutase, along with a decrease in lipid peroxidation and lactate dehydrogenase release.
对一种人工培养的地衣——加特地衣（Usnea ghattensis）的抗氧化和肝脏保护活性进行了研究。人工培养的加特地衣的甲醇提取物表现出良好的抗氧化活性，在20微克/毫升的Trolox等效抗氧化能力下，可防止脂质过氧化67%和86%。它还表现出超氧化物、1,1-二苯基-2-苦基肼、一氧化氮和羟自由基清除活性，分别为89%、89.6%、94.8%和89.6%，并且发现在20微克/毫升浓度下其水平高于已知的合成抗氧化剂丁基羟基甲苯、丁基羟基茴香醚和槲皮素。在小鼠肝脏切片培养模型中，该人工培养的地衣提取物还通过显著降低抗氧化酶、谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶，以及减少脂质过氧化和乳酸脱氢酶释放，表现出对乙醇诱导的毒性的肝脏保护作用。
