Behera B C, Verma Neeraj, Sonone Anjali, Makhija Urmila
Plant Science Division, Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, India.
Microbiol Res. 2006;161(3):232-7. doi: 10.1016/j.micres.2005.08.006. Epub 2005 Sep 19.
The study was aimed to optimize the culture conditions for the production of usnic acid in the cultured cell aggregates composed of symbionts in lichen Usnea ghattensis in vitro. The cultured lichen tissue composed of symbionts appeared after about 2-3 weeks of inoculation in water-agar and malt-yeast extract (MYE) media and shown the production of usnic acid after 2-3 months of inoculation. However, the growth of symbionts was strongly affected by different culture conditions. The addition of excess carbon and nitrogen sources in the media has significantly enhanced the growth as well as usnic acid content. The cultured symbionts in MYE medium having 4% sucrose, 4% polyethyl glycol (PEG) gave 7.63 g dry biomass with 3.9 microg usnic acid/g dry biomass. In water-agar medium having 4% sucrose and 4% PEG gave 3.08 g dry biomass with 1.11 microg usnic acid/g dry biomass. The positive effects of medium on the growth of symbionts and the production of usnic acid are seemed to be due to nutritional factors.
该研究旨在优化体外培养的喜马拉雅松萝地衣共生体细胞聚集体中松萝酸的生产培养条件。由共生体组成的培养地衣组织在接种于水琼脂和麦芽酵母提取物（MYE）培养基中约2 - 3周后出现，并在接种2 - 3个月后显示出松萝酸的产生。然而，共生体的生长受到不同培养条件的强烈影响。培养基中添加过量的碳源和氮源显著促进了生长以及松萝酸含量。在含有4%蔗糖、4%聚乙二醇（PEG）的MYE培养基中培养的共生体产生了7.63 g干生物量，每克干生物量含有3.9 μg松萝酸。在含有4%蔗糖和4% PEG的水琼脂培养基中产生了3.08 g干生物量，每克干生物量含有1.11 μg松萝酸。培养基对共生体生长和松萝酸产生的积极影响似乎归因于营养因素。
