Department of Agroecology, Aarhus University, Slagelse, Denmark.
J Agric Food Chem. 2012 Mar 14;60(10):2497-506. doi: 10.1021/jf2048492. Epub 2012 Mar 6.
Recently, bioactive benzoxazinoids were discovered in cereal grains and bakery products. In this study, we studied the uptake, distribution, and metabolism of these secondary metabolites using a pig model. Twelve benzoxazinoid compounds and their 4 transformation products were quantified in the pigs' diets and biofluids using high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry. The 2-β-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-glc) was the most dominant benzoxazinoid (232 nmol/g DM) seconded by the double-hexose derivative of DIBOA (provisionally characterized here as DIBOA-glc-hex) in the rye-based diet. DIBOA-glc (derived from the diet and intestinal deglycosylation of DIBOA-glc-hex) was apparently reduced to 2-β-D-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-glc), the most dominant benzoxazinoid in the blood (829 nmol/L). The benzoxazinoid compounds were excreted in the urine, with HBOA-glc (18 μmol/L) as a major metabolite. In this study, we determined for the first time the bioavailability of dietary benzoxazinoids that have high digestibility, distribution, and metabolism in mammals. These findings could be a milestone for the exploitation of healthful and pharmacological properties of benzoxazinoids.
最近，在谷物和烘焙食品中发现了具有生物活性的苯并恶嗪类化合物。在这项研究中，我们使用猪模型研究了这些次生代谢物的摄取、分布和代谢。使用高效液相色谱-电喷雾电离三重四极杆质谱联用技术，定量分析了猪饲料和生物体液中的 12 种苯并恶嗪类化合物及其 4 种转化产物。在黑麦基饲料中，2-β-D-吡喃葡萄糖氧基-4-羟基-1,4-苯并恶嗪-3-酮（DIBOA-glc）是最主要的苯并恶嗪类化合物（232 nmol/g DM），其次是 DIBOA 的双己糖衍生物（此处暂定名为 DIBOA-glc-hex）。DIBOA-glc（来源于饮食和 DIBOA-glc-hex 的肠道去糖基化）显然被还原为 2-β-D-吡喃葡萄糖氧基-1,4-苯并恶嗪-3-酮（HBOA-glc），这是血液中最主要的苯并恶嗪类化合物（829 nmol/L）。苯并恶嗪类化合物随尿液排出，其中 HBOA-glc（18 μmol/L）是主要代谢物。在这项研究中，我们首次确定了具有高消化率、分布和代谢的膳食苯并恶嗪类化合物的生物利用度。这些发现可能是开发苯并恶嗪类化合物的健康和药理学特性的一个里程碑。
