Jensen Bettina M, Adhikari Khem B, Schnoor Heidi J, Juel-Berg Nanna, Fomsgaard Inge S, Poulsen Lars K
Allergy Clinic, Gentofte Hospital, Copenhagen University Hospital, dept. 22, 1. Floor, Hellerup, 2900, Gentofte, Denmark.
Department of Agroecology, Aarhus University, Slagelse, Denmark.
Eur J Nutr. 2017 Feb;56(1):387-397. doi: 10.1007/s00394-015-1088-6. Epub 2015 Oct 30.
Benzoxazinoids (BXs) are a group of wholegrain phytochemicals with potential pharmacological properties; however, limited information exists on their absorption, metabolism, and excretion in humans. The aim of this study was to investigate the dose-dependent uptake and excretion of dietary BXs in a healthy population.
Blood and urine were collected from 19 healthy participants from a crossover study after a washout, a LOW BX diet or HIGH BX diet, and analysed for 12 BXs and 4 phenoxazinone derivatives.
We found that the plasma BX level peaked approximately 3 h after food intake, whereas BXs in urine were present even at 36 h after consuming a meal. No phenoxazinone derivatives could be detected in either plasma or urine. The dominant BX metabolite in both plasma and urine was 2-β-D-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-Glc), even though 2-β-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-Glc) was the major component in the diet.
The dietary BX treatment correlated well with the plasma and urine levels, illustrating strong dose-dependent BX absorption, which also had a rapid washout, especially from the plasma compartment.
苯并恶嗪类化合物（BXs）是一类具有潜在药理特性的全谷物植物化学物质；然而，关于它们在人体中的吸收、代谢和排泄的信息有限。本研究的目的是调查健康人群中膳食BXs的剂量依赖性吸收和排泄情况。
在一项交叉研究中，从19名健康参与者身上采集血液和尿液，经过洗脱期、低BX饮食期或高BX饮食期后，对其中的12种BXs和4种吩恶嗪酮衍生物进行分析。
我们发现，血浆中的BX水平在进食后约3小时达到峰值，而尿液中的BXs在进食后36小时仍有存在。在血浆或尿液中均未检测到吩恶嗪酮衍生物。血浆和尿液中主要的BX代谢产物都是2-β-D-吡喃葡萄糖氧基-1,4-苯并恶嗪-3-酮（HBOA-Glc），尽管2-β-D-吡喃葡萄糖氧基-4-羟基-1,4-苯并恶嗪-3-酮（DIBOA-Glc）是饮食中的主要成分。
膳食BX处理与血浆和尿液水平具有良好的相关性，表明BX的吸收具有强烈的剂量依赖性，且清除迅速，尤其是从血浆中清除。
