Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 39, Gothenburg, Sweden.
Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark.
Sci Rep. 2022 Mar 28;12(1):5260. doi: 10.1038/s41598-022-08856-z.
Prostate cancer (PC) is a common cancer among men, and preventive strategies are warranted. Benzoxazinoids (BXs) in rye have shown potential against PC in vitro but human studies are lacking. The aim was to establish a quantitative method for analysis of BXs and investigate their plasma levels after a whole grain/bran rye vs refined wheat intervention, as well as exploring their association with PSA, in men with PC. A quantitative method for analysis of 22 BXs, including novel metabolites identified by mass spectrometry and NMR, was established, and applied to plasma samples from a randomized crossover study where patients with indolent PC (n = 17) consumed 485 g whole grain rye/rye bran or fiber supplemented refined wheat daily for 6 wk. Most BXs were significantly higher in plasma after rye (0.3-19.4 nmol/L in plasma) vs. refined wheat (0.05-2.9 nmol/L) intake. HBOA-glc, 2-HHPAA, HBOA-glcA, 2-HPAA-glcA were inversely correlated to PSA in plasma (p < 0.04). To conclude, BXs in plasma, including metabolites not previously analyzed, were quantified. BX metabolites were significantly higher after rye vs refined wheat consumption. Four BX-related metabolites were inversely associated with PSA, which merits further investigation.
前列腺癌（PC）是男性常见的癌症，需要采取预防策略。黑麦中的苯并恶嗪类（BXs）在体外显示出对 PC 的潜在作用，但缺乏人体研究。本研究旨在建立一种定量分析 BXs 的方法，并研究全谷物/黑麦麸与精制小麦干预后其在血浆中的水平，以及探索它们与 PSA 的关系，在患有 PC 的男性中。建立了一种定量分析 22 种 BXs 的方法，包括通过质谱和 NMR 鉴定的新型代谢物，并将其应用于一项随机交叉研究的血浆样本中，该研究中 17 名惰性 PC 患者每天摄入 485g 全谷物黑麦/黑麦麸或纤维补充精制小麦 6 周。与精制小麦（0.05-2.9nmol/L）相比，黑麦（0.3-19.4nmol/L）摄入后大多数 BXs 在血浆中显著升高。HBOA-glc、2-HHPAA、HBOA-glcA、2-HPAA-glcA 与血浆中的 PSA 呈负相关（p<0.04）。总之，定量分析了血浆中的 BXs，包括以前未分析的代谢物。与精制小麦相比，黑麦摄入后 BX 代谢物显著升高。四种与 BX 相关的代谢物与 PSA 呈负相关，值得进一步研究。
