Pharmaceutical Development of Green Innovations Group, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand.
Int J Pharm. 2012 May 10;427(2):379-84. doi: 10.1016/j.ijpharm.2012.02.010. Epub 2012 Feb 14.
In this study, a blend mixture of chitosan-ethylenediaminetetraacetic acid (CS 2 wt%-EDTA) at a weight ratio of 30/70 and polyvinyl alcohol (PVA) solution (10 wt%) was electrospun to produce fibrous mats with lysozyme (10, 20 and 30 wt%) used for wound healing. The morphology and diameter of the electrospun fiber mats with and without lysozyme were analyzed by scanning electron microscopy (SEM). The amount of lysozyme loaded in the nanofiber mats was measured by HPLC. The cell lysis activity of the lysozyme was investigated with Micrococcus lysodeikticus cells as a substrate. The wound healing activity was performed in vivo using male Wistar rats. The SEM images of all lysozyme-loaded fibers show a smooth fiber without beads with an average diameter of 143-209 nm. The amount of lysozyme loaded in the nanofiber mats was slightly decreased when the initial concentration of lysozyme was increased. The rapid lysozyme release from the nanofiber mats was obtained and is dependent on the lysozyme-loading amount. In animal wound healing, lysozyme loaded CS-EDTA nanofiber mats accelerated the rate of wound healing when compared to the controls (gauze). In conclusion, our experiments demonstrated that biomaterials composed of lysozyme loaded CS-EDTA nanofibers have a potential for wound healing.
在这项研究中，将壳聚糖-乙二胺四乙酸（CS 2wt%-EDTA）与聚乙烯醇（PVA）溶液（10wt%）以 30/70 的重量比混合进行静电纺丝，以生产载有溶菌酶（10、20 和 30wt%）的纤维垫，用于伤口愈合。通过扫描电子显微镜（SEM）分析了载有和未载有溶菌酶的电纺纤维垫的形态和直径。通过 HPLC 测量了纳米纤维垫中溶菌酶的负载量。使用微球菌溶壁酶细胞作为底物研究了溶菌酶的细胞裂解活性。通过雄性 Wistar 大鼠进行体内伤口愈合活性实验。所有载有溶菌酶的纤维的 SEM 图像均显示出无珠的光滑纤维，平均直径为 143-209nm。当溶菌酶的初始浓度增加时，纳米纤维垫中负载的溶菌酶的量略有减少。从纳米纤维垫中快速释放溶菌酶，并且取决于溶菌酶的负载量。在动物伤口愈合中，与对照组（纱布）相比，载有溶菌酶的 CS-EDTA 纳米纤维垫加速了伤口愈合的速度。总之，我们的实验表明，由载有溶菌酶的 CS-EDTA 纳米纤维组成的生物材料具有伤口愈合的潜力。
