State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
FEMS Microbiol Lett. 2012 Nov;336(2):79-88. doi: 10.1111/j.1574-6968.2012.02654.x. Epub 2012 Sep 18.
The anaerobic ammonium-oxidizing (anammox) and nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria in a paddy soil core (0-100 cm) were investigated with newly designed primers targeting the hydrazine synthase β-subunit (hzsB) of anammox bacteria and the recently published primers targeting the pmoA and 16S rRNA genes of n-damo bacteria. The hzsB gene was identified as a proper biomarker to explore the anammox bacterial biodiversity and abundance in soil. The anammox bacteria were present throughout the soil core with the highest abundance of 2.7 × 10(6) hzsB copies g(-1) dry soil at 40-50 cm and were not detectable below 70 cm. Sequences related to at least three species of known anammox bacteria, 'Brocadia anammoxidans', 'Brocadia fulgida', and 'Jettenia asiatica' were detected. By combining the analysis of pmoA and 16S rRNA genes, the n-damo bacteria were observed to be present in 30-70 cm with abundance from 6.5 × 10(3) (60-70 cm) to 7.5 × 10(4) (30-40 cm) copies g(-1) dry soil. The pmoA sequences retrieved from different depths closely related to each other and formed a unique clade. Our results showed that anammox and n-damo bacteria co-occurred in the paddy soil. Both of them were abundant in deep layers (30-60 cm) and the community structures changed along depths in the soil core.
采用新设计的针对厌氧氨氧化菌肼合酶β亚基（hzsB）和最近发表的针对亚硝酸盐依赖型厌氧甲烷氧化菌（n-damo）的 pmoA 和 16S rRNA 基因的引物，对稻田土壤芯（0-100cm）中的厌氧氨氧化（anammox）和亚硝酸盐依赖型厌氧甲烷氧化（n-damo）细菌进行了研究。hzsB 基因被鉴定为探索土壤中厌氧氨氧化菌生物多样性和丰度的合适生物标志物。anammox 细菌存在于整个土壤芯中，在 40-50cm 处的丰度最高，为 2.7×10(6)hzsB 拷贝 g(-1)干土，在 70cm 以下不可检测。检测到至少三种已知厌氧氨氧化菌，即“Brocadia anammoxidans”、“Brocadia fulgida”和“Jettenia asiatica”的相关序列。通过结合 pmoA 和 16S rRNA 基因分析，发现 n-damo 细菌存在于 30-70cm 处，丰度从 6.5×10(3)（60-70cm）到 7.5×10(4)（30-40cm）拷贝 g(-1)干土。从不同深度提取的 pmoA 序列彼此密切相关，形成了一个独特的分支。我们的结果表明，anammox 和 n-damo 细菌共同存在于稻田土壤中。它们都在深层（30-60cm）中丰富，并且在土壤芯的深度上群落结构发生变化。
