Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Infect Immun. 2012 May;80(5):1634-8. doi: 10.1128/IAI.06134-11. Epub 2012 Feb 21.
Recent studies suggest that extracellular DNA promotes biofilm formation in Staphylococcus aureus and, conversely, that extracellular nucleases limit the ability to form a biofilm. S. aureus produces at least two extracellular nucleases, and in the study described in this report, we examined the impact of each of these nucleases on biofilm formation under both in vitro and in vivo conditions. Our results demonstrate that both nucleases impact biofilm formation in the clinical isolate UAMS-1. Under certain in vitro conditions, this impact is negative, with mutation of either or both of the nuclease genes (nuc1 and nuc2) resulting in an enhanced capacity to form a biofilm. However, this effect was not apparent in vivo in a murine model of catheter-associated biofilm formation. Rather, mutation of either or both nuclease genes appeared to limit biofilm formation to a degree that could be correlated with increased susceptibility to daptomycin.
最近的研究表明，细胞外 DNA 可促进金黄色葡萄球菌生物膜的形成，而相反，细胞外核酸酶则限制了形成生物膜的能力。金黄色葡萄球菌至少产生两种细胞外核酸酶，在本报告所述的研究中，我们研究了这两种核酸酶中的每一种在体外和体内条件下对生物膜形成的影响。我们的结果表明，这两种核酸酶都影响临床分离株 UAMS-1 的生物膜形成。在某些体外条件下，这种影响是负面的，突变任一种或两种核酸酶基因（nuc1 和 nuc2）都会导致生物膜形成能力增强。然而，在导管相关生物膜形成的小鼠模型中，这种效应在体内并不明显。相反，任一种或两种核酸酶基因的突变似乎限制了生物膜的形成程度，这与增加对达托霉素的敏感性有关。
