Institut für Molekulare Kardiologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 May-Jun;4(3):329-43. doi: 10.1002/wnan.1163. Epub 2012 Feb 21.
In this article, we review the use of (19) F MRI (magnetic resonance imaging) for in vivo tracking of monocytes and macrophages in the course of tissue inflammation. Emulsified perfluorocarbons (PFCs) are preferentially phagocytized by monocytes/macrophages and are readily detected by (19) F MRI. Because of the lack of any (19) F background in the body, observed signals are robust and exhibit an excellent degree of specificity. As a consequence of progressive infiltration of the labeled immunocompetent cells into inflamed areas, foci of inflammation can be localized as hot spots by simultaneous acquisition of morphologically matched proton ((1) H) and fluorine ((19) F) MRI. The identification of inflammation by (19) F MRI--at a time when the inflammatory cascade is initiated--opens the possibility for an early detection and more timely therapeutic intervention. Since signal intensity in the (19) F images reflects the severity of inflammation, this approach is also suitable to monitor the efficacy of pharmaceutical treatment. Because PFCs are biochemically inert and the fluorine nucleus exhibits high magnetic resonance (MR) sensitivity, (19) F MRI may be applicable for clinical inflammation imaging.
在本文中，我们回顾了（19）F MRI（磁共振成像）在组织炎症过程中用于单核细胞和巨噬细胞体内示踪的应用。乳化全氟碳化物（PFC）优先被单核细胞/巨噬细胞吞噬，并可通过（19）F MRI 轻松检测到。由于体内没有任何（19）F 背景，因此观察到的信号非常稳健，并且具有出色的特异性。由于标记的免疫活性细胞逐渐渗透到炎症区域，因此可以通过同时采集形态匹配的质子（（1）H）和氟（（19）F）MRI 来定位炎症焦点。通过（19）F MRI 对炎症进行识别——在炎症级联反应开始时——为早期检测和更及时的治疗干预提供了可能性。由于（19）F 图像中的信号强度反映了炎症的严重程度，因此这种方法也适用于监测药物治疗的效果。由于 PFC 具有生物化学惰性，并且氟核具有高磁共振（MR）灵敏度，因此（19）F MRI 可能适用于临床炎症成像。
