Dietrich Thore, Bujak Stephan Theodor, Keller Thorsten, Schnackenburg Bernhard, Bourayou Riad, Gebker Rolf, Graf Kristof, Fleck Eckart
Department of Cardiology, Deutsches Herzzentrum Berlin, Berlin 13353, Germany.
Department of Geriatrics, Krankenhaus Hedwigshöhe, Alexianer St. Hedwig Kliniken Berlin GmbH, Berlin 12526, Germany.
Int J Biomed Imaging. 2023 Jul 14;2023:4659041. doi: 10.1155/2023/4659041. eCollection 2023.
The usefulness of perfluorocarbon nanoemulsions for the imaging of experimental myocarditis has been demonstrated in a high-field 9.4 Tesla MRI scanner. Our proof-of-concept study investigated the imaging capacity of PFC-based F/H MRI in an animal myocarditis model using a clinical field strength of 1.5 Tesla. To induce experimental myocarditis, five male rats (weight ~300 g, age ~50 days) were treated with one application per week of doxorubicin (2 mg/kg BW) over a period of six weeks. Three control animals received the identical volume of sodium chloride 0.9% instead. Following week six, all animals received a single 4 ml injection of an 20% oil-in-water perfluorooctylbromide nanoemulsion 24 hours prior to H/F imaging on a 1.5 Tesla MRI. After euthanasia, cardiac histology and immunohistochemistry using CD68/ED1 macrophage antibodies were performed, measuring the inflamed myocardium in m for further statistical analysis to compare the extent of the inflammation with the F-MRI signal intensity. All animals treated with doxorubicin showed a specific signal in the myocardium, while no myocardial signal could be detected in the control group. Additionally, the doxorubicin group showed a significantly higher SNR for F and a stronger CD68/ED1 immunhistoreactivity compared to the control group. This proof-of-concept study demonstrates that perfluorocarbon nanoemulsions could be detected in an experimental myocarditis model at a currently clinically relevant field strength.
全氟碳纳米乳剂在实验性心肌炎成像中的效用已在高场9.4特斯拉MRI扫描仪中得到证实。我们的概念验证研究在动物心肌炎模型中使用1.5特斯拉的临床场强，研究了基于全氟碳的氟/氢MRI的成像能力。为诱导实验性心肌炎，五只雄性大鼠（体重约300克，年龄约50天）在六周内每周接受一次阿霉素（2毫克/千克体重）治疗。三只对照动物改为接受相同体积的0.9%氯化钠。在第六周后，所有动物在1.5特斯拉MRI上进行氢/氟成像前24小时接受一次4毫升的20%水包油型全氟辛基溴纳米乳剂注射。安乐死后，进行心脏组织学检查以及使用CD68/ED1巨噬细胞抗体的免疫组织化学检查，测量炎症心肌的面积（以平方毫米为单位），以便进行进一步的统计分析，将炎症程度与氟MRI信号强度进行比较。所有接受阿霉素治疗的动物心肌均显示出特定信号，而对照组未检测到心肌信号。此外，与对照组相比，阿霉素组的氟信号噪声比显著更高，CD68/ED1免疫组织反应性更强。这项概念验证研究表明，在目前临床相关的场强下，全氟碳纳米乳剂可在实验性心肌炎模型中被检测到。
