School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, PR China.
Biotechnol Bioeng. 2012 Aug;109(8):1958-64. doi: 10.1002/bit.24477. Epub 2012 Mar 2.
A novel green unicellular microalgal isolate from the freshwater of the Inner Mongolia Province of China and named as CCTCC M209220, grows between pH 6 and 11 and temperatures of 20-35°C with optimal conditions at pH 9 and 30°C. Morphological features and the phylogenetic analysis for the 18S rRNA gene reveal that the isolate is a Chlorella sorokiniana strain. A nitrogen source test reveals that this strain can grow well with nitrate and urea, but not ammonium. The strain can grow heterotrophically with glucose as the carbon source and accumulates lipid content as high as 56% (w/w) dry weight after 7 days in high glucose concentrations compared to 19% lipids achieved in 30 days of photoautotrophic culture. The relative neutral lipid content as a fraction of the total lipid is also much higher in heterotrophic culture as compared to photoautotrophic culture.
一种新型的来自中国内蒙古淡水的单细胞微藻分离株，命名为 CCTCC M209220，在 pH 值 6 到 11 和 20-35°C 的温度之间生长，最适条件为 pH 值 9 和 30°C。形态特征和 18S rRNA 基因的系统发育分析表明，该分离株是一种拟球藻属菌株。氮源试验表明，该菌株可以很好地利用硝酸盐和尿素生长，但不能利用铵盐。该菌株可以异养生长，以葡萄糖作为碳源，在高葡萄糖浓度下培养 7 天后，脂质含量可高达 56%（干重），而在 30 天的光自养培养中，脂质含量仅为 19%。与光自养培养相比，异养培养中中性脂质占总脂质的比例也更高。
