National Institute of Molecular Biology and Biotechnology (NIMBB), University of the Philippines, Diliman, Quezon City, Philippines.
National Institute of Molecular Biology and Biotechnology (NIMBB), University of the Philippines, Diliman, Quezon City, Philippines.
Bioresour Technol. 2015 Sep;191:395-8. doi: 10.1016/j.biortech.2015.03.098. Epub 2015 Mar 28.
A local Chlorella sp. isolate with 97% rbcL sequence identity to Chlorella sorokiniana was evaluated in terms of its biomass and lipid production under mixotrophic growth conditions. Glucose-supplemented cultures exhibited increasing growth rate and biomass yield with increasing glucose concentration. Highest growth rate and biomass yield of 1.602 day(-1) and 687.5 mg L(-1), respectively, were achieved under 2 g L(-1) glucose. Nitrogen starvation up to 75% in the 1.0 g L(-1) glucose-supplemented culture was done to induce lipid accumulation and did not significantly affect the growth. Lipid content ranges from 20% to 27% dry weight. Nile Red staining showed more prominent neutral lipid bodies in starved mixotrophic cultures. C. sorokiniana exhibited enhanced biomass production under mixotrophy and more prominent neutral lipid accumulation under nitrogen starvation with no significant decrease in growth; hence, this isolate could be further studied to establish its potential for biodiesel production.
对一株与栅列藻（Chlorella sorokiniana）rbcL 序列同源性达 97%的本地衣藻（Chlorella sp.）分离株进行了研究，评估了其在混合营养生长条件下的生物量和油脂生产能力。补充葡萄糖的培养物表现出随着葡萄糖浓度的增加而增加的生长速率和生物量产量。在 2 g/L 葡萄糖条件下，获得了最高的生长速率和生物量产量，分别为 1.602 天(-1)和 687.5 mg/L。在 1.0 g/L 葡萄糖补充培养物中进行氮饥饿至 75%，以诱导油脂积累，但对生长没有显著影响。脂质含量在干重的 20%至 27%之间。尼罗红染色显示，在饥饿的混合营养培养物中存在更明显的中性脂体。栅列藻在混合营养条件下表现出增强的生物量生产能力，在氮饥饿条件下表现出更明显的中性脂积累，而生长没有明显下降；因此，该分离株可以进一步研究，以确定其用于生物柴油生产的潜力。
