Li Ling Zhao, Wang Zhong Quan, Jiang Peng, Zhang Xi, Ren Hui Jun, Cui Jing
Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, Henan Province, PR China.
Korean J Parasitol. 2011 Dec;49(4):381-4. doi: 10.3347/kjp.2011.49.4.381. Epub 2011 Dec 16.
The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.
1964年中国西藏报告了首例旋毛虫病人类病例。然而，直至目前，旋毛虫病的病原体仍不明确。本研究的目的是通过基于聚合酶链反应（PCR）的方法在种水平上鉴定一株西藏旋毛虫分离株。多重PCR显示，在含有来自自然感染猪的西藏旋毛虫分离株幼虫DNA的检测中，旋毛形线虫出现了预期大小（173 bp）的扩增子。还通过对5S核糖体DNA基因间隔区（5S ISR）和线粒体大亚基核糖体RNA（mt-lsrDNA）基因序列进行PCR扩增来鉴定西藏旋毛虫分离株。结果显示，西藏旋毛虫分离株的2个DNA片段（749 bp和445 bp）与旋毛形线虫参考分离株的片段相同。该西藏旋毛虫分离株可能可归类为旋毛形线虫。这是中国西南部关于旋毛形线虫的首次报道。
