Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, 450052, People's Republic of China.
Parasitol Res. 2012 Feb;110(2):753-7. doi: 10.1007/s00436-011-2549-3. Epub 2011 Jul 20.
Polymerase chain reaction (PCR) and sequencing are useful for species identification of Trichinella spp., especially when their morphological characteristics useful for identifying taxa are lacking. In the present study, nine Trichinella isolates from different provinces in mainland China were identified by the PCR-based method using the 5S ribosomal DNA intergene spacer region (5S ISR) and the mitochondrial large subunit ribosomal DNA genes as molecular markers. The results indicated that eight isolates originating from domestic pigs and one isolate originating from civet cat (Paguma larvata) showed identical DNA banding pattern to Trichinella spiralis. Sequence analysis of the 5S ISR gene further confirmed that the nine Trichinella isolates were T. spiralis and revealed the intraspecies genetic variation within T. spiralis.
聚合酶链反应（PCR）和测序可用于鉴定旋毛虫属的物种，特别是当它们缺乏用于鉴定分类群的形态特征时。在本研究中，使用基于 PCR 的方法，使用 5S 核糖体 DNA 基因间 spacer 区（5S ISR）和线粒体大亚基核糖体 DNA 基因作为分子标记，对来自中国大陆不同省份的 9 株旋毛虫分离株进行了鉴定。结果表明，源自家猪的 8 株和源自灵猫（Paguma larvata）的 1 株分离株与旋毛虫旋毛虫具有相同的 DNA 带型。5S ISR 基因的序列分析进一步证实，这 9 株旋毛虫分离株均为旋毛虫，揭示了旋毛虫属内的种内遗传变异。
