Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Frederick, MD 21702, USA.
Viruses. 2011 Dec;3(12):2442-61. doi: 10.3390/v3122442. Epub 2011 Dec 19.
The cell lines of the NCI-60 panel represent different cancer types and have been widely utilized for drug screening and molecular target identification. Screening these cell lines for envelope proteins or gene sequences related to xenotropic murine leukemia viruses (X-MLVs) revealed that one cell line, EKVX, was a candidate for production of an infectious gammaretrovirus. The presence of a retrovirus infectious to human cells was confirmed by the cell-free transmission of infection to the human prostate cancer cell line LNCaP. Amplification and sequencing of additional proviral sequences from EKVX confirmed a high degree of similarity to X-MLV. The cell line EKVX was established following passage of the original tumor cells through nude mice, providing a possible source of the X-MLV found in the EKVX cells.
NCI-60 面板的细胞系代表不同的癌症类型，已被广泛用于药物筛选和分子靶标鉴定。对这些细胞系进行包膜蛋白或与异嗜性鼠白血病病毒（X-MLVs）相关的基因序列筛查显示，其中一个细胞系 EKVX 是产生感染性γ逆转录病毒的候选细胞系。通过无细胞方式将感染传播到人类前列腺癌细胞系 LNCaP，证实了一种可感染人类细胞的逆转录病毒的存在。从 EKVX 中扩增和测序额外的前病毒序列证实了与 X-MLV 的高度相似性。EKVX 细胞系是在原始肿瘤细胞通过裸鼠传代后建立的，这可能是在 EKVX 细胞中发现的 X-MLV 的来源。
