Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Sci Rep. 2011;1:170. doi: 10.1038/srep00170. Epub 2011 Nov 25.
We present bacterial biogeography as sampled from the human gastrointestinal tract of four healthy subjects. This study generated >32 million paired-end sequences of bacterial 16S rRNA genes (V3 region) representing >95,000 unique operational taxonomic units (OTUs; 97% similarity clusters), with >99% Good's coverage for all samples. The highest OTU richness and phylogenetic diversity was found in the mouth samples. The microbial communities of multiple biopsy sites within the colon were highly similar within individuals and largely distinct from those in stool. Within an individual, OTU overlap among broad site definitions (mouth, stomach/duodenum, colon and stool) ranged from 32-110 OTUs, 25 of which were common to all individuals and included OTUs affiliated with Faecalibacterium prasnitzii and the TM7 phylum. This first comprehensive characterization of the abundant and rare microflora found along the healthy human digestive tract represents essential groundwork to investigate further how the human microbiome relates to health and disease.
我们呈现了从四位健康受试者的胃肠道中采样得到的细菌生物地理学信息。本研究生成了超过 3200 万对细菌 16S rRNA 基因（V3 区）的测序数据，这些数据代表了超过 95000 个独特的操作分类单元（OTUs；相似度为 97%的聚类），所有样本的 Good's 覆盖率均超过 99%。口腔样本中的 OTU 丰富度和系统发育多样性最高。个体内结肠内多个活检部位的微生物群落高度相似，与粪便中的微生物群落有很大的区别。在个体内，广义部位定义（口腔、胃/十二指肠、结肠和粪便）之间的 OTU 重叠范围为 32-110 个 OTUs，其中 25 个 OTUs在所有个体中都存在，包括与普拉梭菌（Faecalibacterium prausnitzii）和 TM7 门相关的 OTUs。这是首次对健康人体消化道中丰富和稀有微生物群的全面描述，为进一步研究人类微生物组与健康和疾病的关系奠定了重要基础。
