Department of Microbiology, Medical Research Institute, University of Alexandria, Alexandria, Egypt.
East Mediterr Health J. 2011 Dec;17(12):925-9. doi: 10.26719/2011.17.12.925.
Earlier reports on the detection of Helicobacter DNA in the gallbladder tissue of patients with biliary diseases have shown discordant results. This study aimed to detect the presence of Helicobacter in gallstone, gallbladder tissue and bile specimens from subjects with H. pylori-positive gastritis with cholelithiasis. The presence of H. pylori in antrum biopsies was confirmed by rapid urease test and/or histopathological examination. DNA was extracted from gallbladder, bile and gallstone samples from 50 patients undergoing cholecystectomy. The presence of Helicobacter genus-specific DNA (16S rRNA genes) was determined by nested polymerase chain reaction assay. Helicobacter DNA was detected in the gallbladder tissue and bile of 28% and 18% respectively of the patients, but was not detected in any of the gallstones. These results do not rule out the possibility of Helicobacter infection as a contributing agent or cofactor in the development of biliary diseases.
先前有报道称，在患有胆病的患者的胆囊组织中检测到了幽门螺杆菌 DNA，但结果并不一致。本研究旨在检测 H. pylori 阳性胃炎合并胆石症患者的胆结石、胆囊组织和胆汁标本中是否存在幽门螺杆菌。通过快速尿素酶试验和/或组织病理学检查确认胃窦活检中是否存在 H. pylori。从 50 名接受胆囊切除术的患者的胆囊、胆汁和胆结石样本中提取 DNA。通过巢式聚合酶链反应检测幽门螺杆菌属特异性 DNA（16S rRNA 基因）。分别在 28%和 18%的患者的胆囊组织和胆汁中检测到了幽门螺杆菌 DNA，但在任何胆结石中均未检测到。这些结果并不能排除幽门螺杆菌感染作为胆病发展的促成因素或协同因素的可能性。
