Bostanoğlu Ebru, Karahan Zeynep Ceren, Bostanoğlu Akın, Savaş Berna, Erden Esra, Kiyan Mehmet
Department of Microbiology and Clinical Microbiology,Ankara University, Faculty of Medicine, Ankara.
Turk J Gastroenterol. 2010 Dec;21(4):421-7. doi: 10.4318/tjg.2010.0130.
BACKGROUND/AIMS: Helicobacter genus and bile-resistant Helicobacter pylori are suggested to have a role in gallstone formation and epithelial cell proliferation in the gallbladder. The aim of this study was to evaluate the presence of Helicobacter species in the gallbladder tissue, bile and gallstones of Turkish patients with cholelithiasis.
Forty-seven patients with calculous cholecystitis and 3 controls were evaluated for the presence of Helicobacter spp. by culture, polymerase chain reaction, and histological and immunohistochemistry methods.
Escherichia coli (10.6%), Enterobacter amnigenus (6.3%), Klebsiella planticola (2.1%), and Klebsiella ozaenae (2.1%) were isolated from the sample cultures of 8 patients. No other microorganisms, including H. pylori and other Helicobacter spp., were detected. Polymerase chain reaction was negative for Helicobacter spp. and H. pylori. No microorganisms resembling Helicobacter spp. were seen on the histological sections. The association between the presence of bacteria and epithelial cell proliferation index was not statistically significant (p=0.48).
There was no association between the presence of Helicobacter spp. and development of cholelithiasis in our study group. The microorganisms found in the samples did not reveal any significant association with the underlying disease.
背景/目的：幽门螺杆菌属及耐胆汁的幽门螺杆菌被认为在胆结石形成及胆囊上皮细胞增殖中发挥作用。本研究旨在评估土耳其胆石症患者胆囊组织、胆汁及胆结石中幽门螺杆菌属的存在情况。
通过培养、聚合酶链反应以及组织学和免疫组织化学方法，对47例结石性胆囊炎患者和3例对照进行幽门螺杆菌属存在情况的评估。
从8例患者的样本培养物中分离出大肠杆菌（10.6%）、产氨肠杆菌（6.3%）、栖稻克雷伯菌（2.1%）和鼻硬结克雷伯菌（2.1%）。未检测到包括幽门螺杆菌和其他幽门螺杆菌属在内的其他微生物。聚合酶链反应检测幽门螺杆菌属和幽门螺杆菌呈阴性。组织学切片上未见类似幽门螺杆菌属的微生物。细菌存在与上皮细胞增殖指数之间的关联无统计学意义（p = 0.48）。
在我们的研究组中，幽门螺杆菌属的存在与胆石症的发生之间无关联。样本中发现的微生物与潜在疾病未显示出任何显著关联。
