Department of Surgery, New York University Langone Medical Center, New York, NY, USA.
HPB (Oxford). 2010 Mar;12(2):129-33. doi: 10.1111/j.1477-2574.2009.00148.x.
This experimental study was designed to determine if Helicobacter spp. contribute to benign gallbladder disease using polymerase chain reaction (PCR) methods.
Patients with benign gallbladder disease scheduled for elective cholecystectomy at New York University Langone Medical Center were recruited from February to May 2008. Bile, gallbladder tissue and gallstones were collected. DNA was isolated from these specimens and amplified via PCR using C97F and C98R primers specific for Helicobacter spp. Appropriate positive and negative controls were used. Products were analysed with agarose gel electrophoresis, sequenced and results aligned using sequencher. Plasma was collected for detection of anti-Helicobacter pylori antibodies via enzyme-linked immunosorbent assay.
Of 36 patients, 12 patients' bile and/or tissue were positive for Helicobacter spp. by PCR. Species were most homologous with H. pylori, although other Helicobacter spp. were suggested. Six of 12 patients demonstrated anti-Helicobacter antibodies in plasma, suggesting that the remaining six might have demonstrated other species besides H. pylori. Four of six plasma samples with anti-Helicobacter antibodies were anti-CagA (cytotoxin associated gene) negative.
Helicobacter spp. can be detected in bile and gallbladder tissue of patients with benign gallbladder disease. The contribution of these bacteria to the pathophysiology of gallbladder disease and gallstone formation requires further study.
本实验研究旨在通过聚合酶链反应（PCR）方法确定螺旋菌是否与良性胆囊疾病有关。
2008 年 2 月至 5 月，从纽约大学朗格尼医学中心接受择期胆囊切除术的良性胆囊疾病患者中招募了研究对象。收集胆汁、胆囊组织和胆结石。从这些标本中提取 DNA，并使用针对螺旋菌的 C97F 和 C98R 引物通过 PCR 进行扩增。使用适当的阳性和阴性对照。通过琼脂糖凝胶电泳分析产物，使用 sequencher 对齐测序结果。收集血浆以通过酶联免疫吸附试验检测抗幽门螺杆菌抗体。
在 36 名患者中，12 名患者的胆汁和/或组织通过 PCR 检测出螺旋菌呈阳性。通过序列分析，这些物种与幽门螺杆菌最同源，但也提示了其他螺旋菌的存在。12 名患者中有 6 名在血浆中显示出抗螺旋菌抗体，这表明其余 6 名患者可能除了幽门螺杆菌外还显示出其他物种。6 份具有抗螺旋菌抗体的血浆样本中有 4 份抗 CagA（细胞毒素相关基因）阴性。
可以在患有良性胆囊疾病的患者的胆汁和胆囊组织中检测到螺旋菌。这些细菌对胆囊疾病和胆结石形成的病理生理学的贡献需要进一步研究。
