Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
J Vet Intern Med. 2012 Mar-Apr;26(2):377-83. doi: 10.1111/j.1939-1676.2012.00890.x. Epub 2012 Feb 22.
Experimental infection of horses with Bartonella species is not documented.
Determine clinical signs, hematologic changes, duration of bacteremia, and pattern of seroconversion in Bartonella henselae or Bartonella bovis-inoculated horses.
Twelve (2 groups of 6) randomly selected healthy adult horses seronegative and culture negative for Bartonella spp.
Experimental/observational study: Group I: B. henselae or saline control was inoculated intradermally into 4 naïve and 2 sentinel horses, respectively. Group II: same design was followed by means of B. bovis. Daily physical examinations, once weekly CBC, immunofluorescent antibody assay serology, real-time polymerase chain reaction (PCR), and twice weekly blood cultures were performed for 6 weeks and at postinoculation day 80 and 139. Bartonella alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture was performed for horses that seroconverted to B. henselae antigens.
Transient clinical signs consistent with bartonellosis occurred in some Bartonella-inoculated horses, but hematological alterations did not occur. Three B. henselae-inoculated horses seroconverted, whereas 1 B. bovis-inoculated horse was weakly seropositive. In Group I, B. henselae was amplified and sequenced from BAPGM blood culture as well as a subculture isolate from 1 horse, blood from a 2nd horse, and BAPGM blood culture from a 3rd horse although a subculture isolate was not obtained. All sentinels remained PCR, culture, and serology negative.
Detection of Bartonella sp. in blood after experimental inoculation supports bacteremia and seroconversion. Culture with BAPGM may be required to detect Bartonella sp. Although mild clinical signs followed acute infection, no long-term effects were noted for 2 years postinoculation.
尚无关于贝氏巴尔通体物种感染马匹的实验记录。
确定接种贝氏巴尔通体或牛氏巴尔通体的马匹的临床症状、血液学变化、菌血症持续时间和血清转化模式。
12 匹（2 组，每组 6 匹）随机选择的健康成年马，血清学检测和培养均为贝氏巴尔通体 spp 阴性。
实验/观察性研究：第 1 组：分别将贝氏巴尔通体或生理盐水对照物皮内接种到 4 匹初免马和 2 匹哨兵马体内。第 2 组：采用相同设计接种牛氏巴尔通体。在接种后 6 周内，每周进行 1 次体格检查、1 次全血细胞计数（CBC）、免疫荧光抗体检测血清学、实时聚合酶链反应（PCR）和每周 2 次血液培养，在接种后第 80 天和 139 天进行检测。对已针对贝氏巴尔通体抗原产生血清转化的马匹进行贝氏α变形菌生长培养基（BAPGM）血液培养以富集巴尔通体。
一些接种了巴尔通体的马匹出现了与巴尔通体病相符的短暂临床症状，但未发生血液学改变。3 匹接种了贝氏巴尔通体的马出现了血清转化，而 1 匹接种了牛氏巴尔通体的马呈弱阳性血清反应。在第 1 组中，从 1 匹马的 BAPGM 血培养以及从 1 匹马的亚培养分离物、1 匹马的血液和第 3 匹马的 BAPGM 血培养中扩增并测序出了贝氏巴尔通体，尽管未获得亚培养分离物。所有哨兵均为 PCR、培养和血清学阴性。
实验接种后血液中检测到巴尔通体支持菌血症和血清转化。可能需要使用 BAPGM 进行培养以检测巴尔通体。尽管急性感染后出现了轻微的临床症状，但在接种后 2 年内未观察到长期影响。
