University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham B18 7QH, UK.
Eur J Clin Invest. 2012 Aug;42(8):832-9. doi: 10.1111/j.1365-2362.2012.02656.x. Epub 2012 Feb 22.
Monocytes are important mediators in the pathophysiology of cardiovascular disease, but only scarce data are available on biological and methodological factors affecting their levels.
Three monocyte subsets, CD14(++) CD16(-) CCR2+ (Mon1), CD14(++) CD16(+) CCR2(+) (Mon2), CD14(+) CD16(+) CCR2(-) (Mon3), and monocyte-platelet aggregates (MPAs) were analysed by flow cytometry. The effects of treadmill exercise were assessed on 12 healthy volunteers. Diurnal variation was evaluated in 16 healthy volunteers, and the effects of delayed blood processing were measured in 12 samples.
Mon1 were increased when measured 15 min after exercise followed by a reduction at 1 h (P < 0·05 for both). MPAs were significantly reduced at 15 min and 1 h (P < 0·05 for both). There was significant diurnal variation in the numbers of Mon2, which were highest at 6 pm and lowest at 6 am. There were also significant diurnal variations in phagocytic activity of Mon1 and Mon2, which were highest at 12 pm and lowest at 12 am. Monocyte counts remained stable up to 2 h after venipuncture. MPAs were significantly increased at 2 h and increased further by 4 h after sampling.
Monocyte subset Mon2 and monocyte phagocytic activity undergo significant diurnal variation. A single bout of exercise causes a temporal increase in monocytes and a reduction in MPAs. Monocyte subset counts should be analysed within 2 h of blood sampling, whereas measurement of MPAs and monocyte CD14 and CD16 expression should be performed within 1 h.
单核细胞是心血管疾病病理生理学中的重要介质，但关于影响其水平的生物学和方法学因素的数据却很少。
通过流式细胞术分析了三种单核细胞亚群，即 CD14(++) CD16(-) CCR2+(Mon1)、CD14(++) CD16(+) CCR2(+) (Mon2)、CD14(+) CD16(+) CCR2(-) (Mon3)和单核细胞-血小板聚集体（MPAs）。评估了 12 名健康志愿者的跑步机运动效应。在 16 名健康志愿者中评估了昼夜变化，在 12 个样本中测量了延迟血液处理的影响。
运动后 15 分钟测量时 Mon1 增加，随后 1 小时减少（两者均 P < 0·05）。MPAs 在 15 分钟和 1 小时时显著减少（两者均 P < 0·05）。Mon2 的数量存在显著的昼夜变化，其在下午 6 点最高，早上 6 点最低。Mon1 和 Mon2 的吞噬活性也存在显著的昼夜变化，其在中午 12 点最高，凌晨 12 点最低。采血后 2 小时内单核细胞计数保持稳定。MPAs 在 2 小时时显著增加，在取样后 4 小时进一步增加。
单核细胞亚群 Mon2 和单核细胞吞噬活性存在显著的昼夜变化。一次性运动导致单核细胞暂时增加和 MPAs 减少。单核细胞计数应在采血后 2 小时内分析，而 MPAs 和单核细胞 CD14 和 CD16 表达的测量应在 1 小时内进行。
