Center/Departament of Physics, University of Minho, Campus de Gualtar, Braga, Portugal.
Biomed Mater. 2012 Jun;7(3):035004. doi: 10.1088/1748-6041/7/3/035004. Epub 2012 Feb 23.
Due to the large potential of electroactive materials in novel tissue engineering strategies, the aim of this work is to determine if the crystalline phase and/or the surface electrical charge of electroactive poly(vinylidene fluoride), PVDF, have influence on the biological response in monolayer cell culture. Non-polar α-PVDF and electroactive β-PVDF were prepared. The β-PVDF films were poled by corona discharge to show negative or positive electrical surface charge density. It has been concluded that hydrophilicity of the PVDF substrates depends significantly on crystalline phase and polarity. Furthermore, by means of atomic force microscopy and an enzyme-linked immunosorbent assay test, it has been shown that positive or negative poling strongly influences the behavior of β-PVDF supports with respect to fibronectin (FN) adsorption, varying the exhibition of adhesion ligands of adsorbed FN. Culture of MC3T3-E1 pre-osteoeblasts proved that cell proliferation depends on surface polarity as well. These results open the viability of cell culture stimulation by mechanical deformation of a piezoelectric substrate that results in varying electrical charge densities on the substrate surface.
由于电活性材料在新型组织工程策略中有很大的潜力，本工作旨在确定电活性聚偏二氟乙烯（PVDF）的结晶相和/或表面电荷是否对单层细胞培养中的生物学反应有影响。制备了非极性的α-PVDF 和电活性的β-PVDF。β-PVDF 薄膜通过电晕放电极化，显示出负或正的表面电荷密度。结果表明，PVDF 基底的亲水性显著依赖于结晶相和极性。此外，通过原子力显微镜和酶联免疫吸附试验表明，正或负的极化强烈影响β-PVDF 支架对纤维连接蛋白（FN）吸附的行为，改变吸附 FN 的粘附配体的表现。MC3T3-E1 前成骨细胞的培养证明，细胞增殖也依赖于表面极性。这些结果为通过压电基底的机械变形来刺激细胞培养的可行性开辟了道路，这种变形会导致基底表面的电荷密度发生变化。
