Donnelly Hannah, Sprott Mark R, Poudel Anup, Campsie Paul, Childs Peter, Reid Stuart, Salmerón-Sánchez Manuel, Biggs Manus, Dalby Matthew J
Centre for the Cellular Microenvironment, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, Galway H91W2TY, Ireland.
ACS Appl Mater Interfaces. 2023 Sep 17;15(44):50652-62. doi: 10.1021/acsami.3c05128.
There is an unmet clinical need to provide viable bone grafts for clinical use. Autologous bone, one of the most commonly transplanted tissues, is often used but is associated with donor site morbidity. Tissue engineering strategies to differentiate an autologous cell source, such as mesenchymal stromal cells (MSCs), into a potential bone-graft material could help to fulfill clinical demand. However, osteogenesis of MSCs can typically require long culture periods that are impractical in a clinical setting and can lead to significant cost. Investigation into strategies that optimize cell production is essential. Here, we use the piezoelectric copolymer poly(vinylidene fluoride-trifluoroethylene) (PVDF-TrFE), functionalized with a poly(ethyl acrylate) (PEA) coating that drives fibronectin network formation, to enhance MSC adhesion and to present growth factors in the solid phase. Dynamic electrical cues are then incorporated, via a nanovibrational bioreactor, and the MSC response to electromechanical stimulation is investigated.
提供用于临床的可行骨移植材料存在未满足的临床需求。自体骨是最常用的移植组织之一，经常被使用，但会引发供体部位的并发症。将自体细胞来源（如间充质基质细胞 (MSC)）分化为潜在骨移植材料的组织工程策略有助于满足临床需求。然而，MSC 的成骨通常需要较长的培养时间，这在临床环境中不切实际，并且会导致高昂的成本。研究优化细胞生产的策略至关重要。在这里，我们使用经聚（丙烯酸乙酯）(PEA) 涂层功能化的压电共聚物聚（偏二氟乙烯 - 三氟乙烯）(PVDF-TrFE)，该涂层可驱动纤连蛋白网络形成，以增强 MSC 的粘附并在固相中呈现生长因子。然后通过纳米振动生物反应器引入动态电信号，并研究 MSC 对机电刺激的反应。
