Departamento de Física de la Materia Condensada, C-3, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Ultramicroscopy. 2012 Mar;114:56-61. doi: 10.1016/j.ultramic.2012.01.007. Epub 2012 Jan 20.
Control and minimization of tip-sample interaction forces are imperative tasks to maximize the performance of atomic force microscopy. In particular, when imaging soft biological matter in liquids, the cantilever dragging force prevents identification of the tip-sample mechanical contact, resulting in deleterious interaction with the specimen. In this work we present an improved jumping mode procedure that allows detecting the tip-sample contact with high accuracy, thus minimizing the scanning forces (-100 pN) during the approach cycles. To illustrate this method we report images of human adenovirus and T7 bacteriophage particles which are prone to uncontrolled modifications when using conventional jumping mode.
控制和最小化尖端-样品相互作用力对于最大限度地提高原子力显微镜的性能至关重要。特别是，在液体中对软生物物质成像时，悬臂拖拽力会阻止识别尖端-样品机械接触，从而对样品产生有害的相互作用。在这项工作中，我们提出了一种改进的跳跃模式程序，该程序可以高精度地检测尖端-样品接触，从而在接近循环期间最小化扫描力（-100 pN）。为了说明这种方法，我们报告了人类腺病毒和 T7 噬菌体颗粒的图像，当使用传统的跳跃模式时，这些颗粒容易受到不受控制的修饰。
