Department of Chemical and Biological Engineering, University of Colorado, Campus Box 424, Boulder, CO 80309, USA.
Curr Opin Biotechnol. 2012 Oct;23(5):666-71. doi: 10.1016/j.copbio.2012.01.012. Epub 2012 Feb 20.
Advances in DNA sequencing and synthesis technologies concurrent with the development of new recombinant DNA approaches have enabled the extension of directed evolution algorithms to the genome-scale. It is now possible to simultaneously map the effect of mutation(s) in each and every gene in the genome onto almost any screenable or selectable phenotype in less than a week. Such maps can be used to direct the design and construction of libraries containing billions of rationally designed combinatorial mutations. Such combinatorial libraries can now also be created and evaluated in less than a week. The review presents and discusses these new technologies within the context of directed evolution and inverse metabolic engineering.
DNA 测序和合成技术的进步与新的重组 DNA 方法的发展并行，使定向进化算法得以扩展到基因组规模。现在，在不到一周的时间内，就可以同时映射基因组中每个基因的突变对几乎任何可筛选或可选择表型的影响。这些图谱可用于指导设计和构建包含数十亿个合理设计的组合突变的文库。现在，这些组合文库也可以在不到一周的时间内创建和评估。本文在定向进化和反向代谢工程的背景下介绍和讨论了这些新技术。
