Bioengineered. 2012 Jul-Aug;3(4):218-21. doi: 10.4161/bioe.20431. Epub 2012 Jun 18.
Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.
代谢工程通常通过将来自各种异源生物体的编码酶的基因克隆到大肠杆菌中来实现。这种工程努力经常受到外来基因的阻碍，这些基因对大肠杆菌宿主有毒。我们开发了 PanDaTox（www.weizmann.ac.il/pandatox），这是一个基于网络的资源，提供了来自数百种不同微生物基因组的超过 150 万个基因的实验毒性信息。毒性预测经过了广泛的实验验证，其基础是将基因作为桑格全基因组鸟枪法测序过程的一部分连续克隆到大肠杆菌中。PanDaTox 可以通过允许研究人员从工程计划中排除有毒基因，并在进行实际的代谢工程实验之前验证所选基因的可克隆性，从而加速代谢工程项目。
