Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa 50011 USA.
Am J Bot. 2012 Mar;99(3):e91-3. doi: 10.3732/ajb.1100421. Epub 2012 Feb 21.
Microsatellite primers previously developed for domesticated cotton (Gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within G. davidsonii and five other wild, Mexican, D-genome cotton species (all diploid).
We screened 50 microsatellite primer pairs from the Cotton Marker Database, identifying 10 loci as polymorphic within G. davidsonii. In genotyping approximately 200 individuals from four populations, we found that the number of alleles per locus ranged from seven to 17, and mean observed and expected heterozygosity ranged from 0.145 to 0.492 and from 0.436 to 0.734, respectively. We genotyped six to 20 individuals from each of the remaining species, finding these 10 loci to cross-amplify in all cases and in most cases to be polymorphic.
These markers may be useful for further investigation of population genetics of G. davidsonii and other wild D-genome cotton species.
先前为栽培棉（四倍体棉属）开发的微卫星引物被筛选用于研究 G. davidsonii 及其它五个野生、墨西哥、D 基因组棉种（均为二倍体）内的遗传结构和基因流。
我们从 Cotton Marker Database 中筛选了 50 对微卫星引物，鉴定出 10 个在 G. davidsonii 内多态性的位点。在对来自四个种群的大约 200 个个体进行基因分型时，我们发现每个位点的等位基因数从 7 到 17 不等，平均观察到和预期的杂合度分别从 0.145 到 0.492 和从 0.436 到 0.734 不等。我们对每个剩余物种的 6 到 20 个个体进行了基因分型，发现这 10 个位点在所有情况下都能扩增，并在大多数情况下都具有多态性。
这些标记可能对进一步研究 G. davidsonii 和其他野生 D 基因组棉种的种群遗传学有用。
