Biology Department, Washington University, 1 Brookings Drive, Campus Box 1137, St. Louis, Missouri 63130 USA.
Am J Bot. 2012 Mar;99(3):e111-3. doi: 10.3732/ajb.1100457. Epub 2012 Feb 21.
We developed and characterized microsatellite markers for Byrsonima crassifolia (Malpighiaceae), a widely distributed neotropical fruit tree.
Eight polymorphic and two monomorphic microsatellite loci were identified and screened in 60 samples from four geographically disparate populations (Bolivia, Brazil, Mexico, and Panama). Each locus exhibited between two and 11 alleles. Expected heterozygosity ranged from 0 to 0.839. All loci amplify in the congeners B. variabilis and B. basiloba, four amplify in B. bucidaefolia, and seven amplify in B. variabilis, although levels of polymorphism have not been assessed.
These loci will provide novel tools for comparing genetic diversity present in cultivated and noncultivated populations of B. crassifolia throughout its range, and may prove valuable in related species.
我们开发并鉴定了桃金娘科（Malpighiaceae）广泛分布的热带果树巴尔沙果（Byrsonima crassifolia）的微卫星标记。
从分布于玻利维亚、巴西、墨西哥和巴拿马四个地理上不同的种群的 60 个样本中鉴定并筛选出 8 个多态性和 2 个单态性微卫星位点。每个位点显示出 2 到 11 个等位基因。预期杂合度范围从 0 到 0.839。所有位点都在同属的巴尔沙果（B. variabilis）和毛叶巴尔沙果（B. basiloba）中扩增，4 个在 B. bucidaefolia 中扩增，7 个在 B. variabilis 中扩增，尽管尚未评估其多态性水平。
这些位点将为比较其分布范围内栽培和非栽培巴尔沙果种群中的遗传多样性提供新的工具，并且可能对相关物种具有重要价值。
