State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
School of Biological and Physical Sciences (SBPS), Main Campus, Jaramogi Oginga Odinga University of Science and Technology (JOOUST), Main Campus, P.O. Box 210-40601 Bondo, Kenya.
Int J Mol Sci. 2018 Jan 11;19(1):204. doi: 10.3390/ijms19010204.
The challenge in tetraploid cotton cultivars is the narrow genetic base and therefore, the bottleneck is how to obtain interspecific hybrids and introduce the germplasm directly from wild cotton to elite cultivars. Construction of genetic maps has provided insight into understanding the genome structure, interrelationships between organisms in relation to evolution, and discovery of genes that carry important agronomic traits in plants. In this study, we generated an interspecific hybrid between two wild diploid cottons, and , and genotyped 188 F populations in order to develop a genetic map. We screened 12,560 SWU Simple Sequence Repeat (SSR) primers and obtained 1000 polymorphic markers which accounted for only 8%. A total of 928 polymorphic primers were successfully scored and only 728 were effectively linked across the 13 chromosomes, but with an asymmetrical distribution. The map length was 1480.23 cM, with an average length of 2.182 cM between adjacent markers. A high percentage of the markers on the map developed, and for the physical map of exhibited highly significant collinearity, with two types of duplication. High level of segregation distortion was observed. A total of 27 key genes were identified with diverse roles in plant hormone signaling, development, and defense reactions. The achievement of developing the F population and its genetic map constructions may be a landmark in establishing a new tool for the genetic improvement of cultivars from wild plants in cotton. Our map had an increased recombination length compared to other maps developed from other D genome cotton species.
四倍体棉花品种面临的挑战是遗传基础狭窄，因此，瓶颈在于如何获得种间杂种，并将野生棉的种质资源直接引入到优良品种中。遗传图谱的构建为理解基因组结构、生物体之间的相互关系以及与进化有关的关系以及发现携带植物重要农艺性状的基因提供了深入的了解。在这项研究中，我们生成了两个野生二倍体棉花 和 的种间杂种，并对 188 个 F1 群体进行了基因型分析，以构建遗传图谱。我们筛选了 12560 个 SWU 简单重复序列（SSR）引物，获得了 1000 个多态性标记，仅占 8%。总共成功标记了 928 个多态性引物，并且只有 728 个在 13 条染色体上有效连接，但分布不对称。图谱长度为 1480.23cM，相邻标记之间的平均长度为 2.182cM。图谱上标记的比例很高，并且 物理图谱与两种类型的重复具有高度显著的共线性。观察到高度的分离失真。总共鉴定了 27 个关键基因，它们在植物激素信号转导、发育和防御反应中具有不同的作用。成功构建了 F1 群体及其遗传图谱的建立可能是为棉花野生植物品种的遗传改良建立新工具的一个里程碑。与其他从其他 D 基因组棉花物种开发的图谱相比，我们的图谱增加了重组长度。
