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开发并鉴定了青冈(壳斗科)的多态微卫星标记。

Development and characterization of polymorphic microsatellite markers in Cyclobalanopsis glauca (Fagaceae).

机构信息

School of Resources and Environmental Sciences, Tiantong National Observation Station for Forest Ecosystems, East China Normal University, Shanghai 200062, People's Republic of China.

出版信息

Am J Bot. 2012 Mar;99(3):e120-2. doi: 10.3732/ajb.1100448. Epub 2012 Feb 21.

Abstract

PREMISE OF THE STUDY

We isolated and characterized polymorphic microsatellite loci in Cyclobalanopsis glauca (Fagaceae), an evergreen broadleaved monoecious tree, to provide tools for analyzing parentage and mating system.

METHODS AND RESULTS

Thirteen polymorphic microsatellite markers were developed and tested in three C. glauca populations. The number of alleles per locus varied from two to 22. The observed and expected heterozygosities within populations were 0.000-0.967 and 0.033-0.949, respectively.

CONCLUSIONS

These polymorphic primers showed high levels of polymorphism within tested populations, and can be used in parentage analysis and mating system estimation of C. glauca.

摘要

研究前提

本研究从壳斗科常绿阔叶雌雄同株青冈属中分离并鉴定出多态性微卫星位点,为分析亲代和交配系统提供工具。

方法与结果

在三个青冈属群体中,开发并测试了 13 个多态性微卫星标记。每个位点的等位基因数从两个到 22 个不等。群体内的观察和预期杂合度分别为 0.000-0.967 和 0.033-0.949。

结论

这些多态性引物在测试群体中表现出高水平的多态性,可用于青冈属的亲子分析和交配系统估计。

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