Liu De-Chen, Zhang Yang, Wang Si-Si, Liao Meng-Yu, Fan Xin-Yu, Li Yuan-Yuan, Wang Rong
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Appl Plant Sci. 2017 Jan 5;5(1). doi: 10.3732/apps.1600106. eCollection 2017 Jan.
To estimate the genetic variation of (Ericaceae), a monoecious evergreen shrub, 23 microsatellite markers were identified from its nuclear genome.
We developed 16 polymorphic and seven monomorphic microsatellite primers using the biotin-streptavidin capture method. The 16 polymorphic loci were investigated further using 89 individuals sampled from three populations in China. The number of alleles per locus ranged from four to 30, indicating a high level of polymorphism. The observed heterozygosity varied from 0.1034 to 0.9333, while the expected heterozygosity ranged from 0.1016 to 0.9542. Of these polymorphic primers, 12 were found to be functional in , a congeneric species of .
Moderate to high levels of genetic variation were found in these microsatellite loci, indicating that they can be applied in future studies of genetic structure, contributing to forest management and conservation.
为了估计雌雄同株常绿灌木（杜鹃花科）的遗传变异，从其核基因组中鉴定出23个微卫星标记。
我们使用生物素 - 链霉亲和素捕获法开发了16个多态性微卫星引物和7个单态性微卫星引物。利用从中国三个种群采集的89个个体对这16个多态性位点进行了进一步研究。每个位点的等位基因数从4到30不等，表明多态性水平较高。观察到的杂合度在0.1034至0.9333之间，而预期杂合度在0.1016至0.9542之间。在这些多态性引物中，发现有12个在该属的同属物种中具有功能。
在这些微卫星位点中发现了中度至高程度的遗传变异，表明它们可应用于未来该属的遗传结构研究，有助于森林管理和保护。
