Food and Bioprocess Engineering Group, Wageningen Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands.
Cytotechnology. 1996 Jan;20(1-3):221-9. doi: 10.1007/BF00350402.
In this chapter we have attempted to evaluate the most important parameters which can be useful for the pur-pose of design and scale up. Insect cells and animal cells in general can be grown well in large vessels. However, none of the theories and parameters discussed in this chapter have been validated on a larger scale than laboratory and small pilot reactors. Selection of the most suitable design and scale-up method there-fore needs in particular studies in larger vessels. The Kolmogorov theory and the killing-volume model are in this respect the most promising approaches for the optimal design of large-scale animal-cell bioreactors.
在本章中，我们试图评估对于设计和放大目的而言最重要的参数。昆虫细胞和一般动物细胞都可以在大型容器中很好地生长。然而，本章中讨论的理论和参数都没有在比实验室和小型中试反应器更大的规模上得到验证。因此，最合适的设计和放大方法的选择需要在更大的容器中进行特别研究。在这方面，Kolmogorov 理论和杀灭体积模型是用于大型动物细胞生物反应器的最佳设计的最有前途的方法。
