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本文引用的文献

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Interactions between animal cells and gas bubbles: The influence of serum and pluronic F68 on the physical properties of the bubble surface.动物细胞与气泡之间的相互作用：血清和普朗尼克F68对气泡表面物理性质的影响。
Biotechnol Bioeng. 1994 Mar 15;43(6):446-54. doi: 10.1002/bit.260430603.
2
Quantification of damage to suspended insect cells as a result of bubble rupture.对气泡破裂导致的悬浮昆虫细胞损伤进行定量分析。
Biotechnol Bioeng. 1994 Jan 5;43(1):37-45. doi: 10.1002/bit.260430106.
3
Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of a hybridoma cell in a continuous culture.稀释率对连续培养中杂交瘤细胞生长、生产力、细胞周期与大小以及剪切敏感性的影响。
Biotechnol Bioeng. 1993 Feb 20;41(4):429-39. doi: 10.1002/bit.260410406.
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Hydrodynamic shear stress and mass transport modulation of endothelial cell metabolism.流体切应力和质量传递对血管内皮细胞代谢的调节作用。
Biotechnol Bioeng. 1991 Sep;38(6):588-602. doi: 10.1002/bit.260380605.
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Physical modeling of animal cell damage by hydrodynamic forces in suspension cultures.悬浮培养中流体力学对动物细胞损伤的物理建模。
Biotechnol Bioeng. 1992 Dec 5;40(10):1277-81. doi: 10.1002/bit.260401018.
6
Effect of serum concentration on hybridoma viable cell density and production of monoclonal antibodies in CSTRs and on shear sensitivity in air-lift loop reactors.在 CSTR 和空气升力环式反应器中，血清浓度对杂交瘤活细胞密度和单克隆抗体生产的影响及其对剪切敏感性的影响。
Biotechnol Bioeng. 1992 Apr 15;39(9):891-7. doi: 10.1002/bit.260390902.
7
Agitation induced cell injury in microcarrier cultures. Protective effect of viscosity is agitation intensity dependent: Experiments and modeling.微载体培养中的搅拌诱导细胞损伤。粘度的保护作用与搅拌强度有关：实验与模拟。
Biotechnol Bioeng. 1992 Jan 5;39(1):95-107. doi: 10.1002/bit.260390114.
8
Protection mechanisms of freely suspended animal cells (CRL 8018) from fluid-mechanical injury. Viscometric and bioreactor studies using serum, pluronic F68 and polyethylene glycol.悬浮培养动物细胞（CRL 8018）免受流体机械损伤的保护机制。使用血清、普朗尼克F68和聚乙二醇的粘度测定及生物反应器研究。
Biotechnol Bioeng. 1991 Jun 20;38(2):169-80. doi: 10.1002/bit.260380209.
9
Lethal events during gas sparging in animal cell culture.在动物细胞培养中鼓泡通气时发生的致死事件。
Biotechnol Bioeng. 1991 Mar 5;37(5):484-90. doi: 10.1002/bit.260370510.
10
Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment.有和无气泡夹带情况下搅拌式生物反应器中悬浮动物细胞的损伤机制。
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固体微载体和大孔微载体上生长的vero 细胞在小型空气提升环式反应器中的死亡率。

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.

机构信息

Department of Food Science, Food and Bioengeneering Group, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.

出版信息

Cytotechnology. 1997 Jan;23(1-3):61-75. doi: 10.1023/B:CYTO.0000010399.23373.65.

DOI:10.1023/B:CYTO.0000010399.23373.65
PMID:22358522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449866/
Abstract

The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

摘要

研究了在小型气升式环流反应器中，作为气体流速函数的 Cytodex-3 微载体上生长的 Vero 细胞的死亡率。死亡率可以用一级死亡速率动力学来描述。从活细胞减少、死细胞增加和 LDH 活性增加计算得出的一级死亡速率常数与气体流速呈线性比例关系，具有约 2.10(-3)m(3)液体/m(3)气泡的特定假设致死体积，其中所有细胞均被杀死。此外，在相同的气升式反应器中进行了使用在多孔 Asahi 微载体内部生长的 Vero 细胞的实验。从该实验计算出的特定假设致死体积的值为 3.10(-4)m(3)液体/m(3)气泡，这表明多孔微载体至少部分能够保护细胞免受气泡产生的有害流体动力的影响。