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固体微载体和大孔微载体上生长的vero 细胞在小型空气提升环式反应器中的死亡率。

Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.

机构信息

Department of Food Science, Food and Bioengeneering Group, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.

出版信息

Cytotechnology. 1997 Jan;23(1-3):61-75. doi: 10.1023/B:CYTO.0000010399.23373.65.

DOI:10.1023/B:CYTO.0000010399.23373.65

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449866/

Abstract

The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10(-3)m(3) liquid per m(3) of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10(-4)m(3) liquid per m(3) of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.

摘要

研究了在小型气升式环流反应器中，作为气体流速函数的 Cytodex-3 微载体上生长的 Vero 细胞的死亡率。死亡率可以用一级死亡速率动力学来描述。从活细胞减少、死细胞增加和 LDH 活性增加计算得出的一级死亡速率常数与气体流速呈线性比例关系，具有约 2.10(-3)m(3)液体/m(3)气泡的特定假设致死体积，其中所有细胞均被杀死。此外，在相同的气升式反应器中进行了使用在多孔 Asahi 微载体内部生长的 Vero 细胞的实验。从该实验计算出的特定假设致死体积的值为 3.10(-4)m(3)液体/m(3)气泡，这表明多孔微载体至少部分能够保护细胞免受气泡产生的有害流体动力的影响。