Department of Food Science, Food and Bioengeneering Group, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.
Cytotechnology. 1996 Jan;21(1):45-59. doi: 10.1007/BF00364836.
The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is linear proportional to the gas flow rate, with a specific hypothetical killing volume in which all cells are killed of about 2·10(-3) m(3) liquid per m(3) of air bubbles. In addition, an experiment was conducted in the same air-lift reactor with Vero cells grown inside porous Asahi microcarriers. The specific hypothetical killing volume calculated from this experiment has a value of 3·10(-4) m(3) liquid per m(3) of air bubbles, which shows that the porous microcarriers were at least in part able to protect the cells against the detrimental hydrodynamic forces generated by the bubbles.
考察了在小型气升式环流反应器中，作为气体流速函数的微载体 Cytodex-3 上生长的vero 细胞的死亡率。死亡率可用一级死亡动力学来描述。从活细胞减少、死细胞增加和 LDH 活性增加计算得出的一级死亡速率常数与气体流速呈线性比例，在假设的特定杀灭体积中，所有细胞均被杀死，约为 2·10(-3)m(3)液体/ m(3)气泡。此外，还在相同的气升式反应器中用在多孔 Asahi 微载体内部生长的 vero 细胞进行了实验。从该实验计算出的特定假设杀灭体积的值为 3·10(-4)m(3)液体/ m(3)气泡，这表明多孔微载体至少部分能够保护细胞免受气泡产生的有害流体动力的影响。
