使用具有多沉降区的灌注培养装置高密度培养鼠-人杂交瘤细胞，以将细胞与培养基分离。

High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium.

机构信息

Biotechnology Research Laboratories, Tokyo Research Center, Teijin Limited, 4-3-2, Asahigaoka, 191, Hino-shi, Tokyo, Japan.

出版信息

Cytotechnology. 1989 Feb;2(1):5-8. doi: 10.1007/BF00365409.

DOI:10.1007/BF00365409

PMID:22358575

Abstract

Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×10(7) cells/ml, when the specific perfusion rate was set to 2.3 vol day(-1), and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.

摘要

采用具有三个沉降区的新型灌注培养装置培养鼠-人杂交瘤 X87X 细胞，通过重力沉降将细胞与培养基分离。当特定的灌注率设定为 2.3 体积/天时，在无血清培养基中可达到 3.0×10(7)个细胞/ml 的最大活细胞密度，并且连续生产单克隆抗体。这些结果几乎与具有一个沉降区的灌注培养容器中的结果相同，表明具有多个沉降区的过程是一种很有前途的用于放大重力型灌注培养容器的方法。