Istituto di Anatomia Umana Normale, Bologna, Italy.
Cytotechnology. 1989 Feb;2(1):59-62. doi: 10.1007/BF00365415.
A flow cytometric methodology was set up to assess the binding capability of peripheral blood NK and T cells to the K562 tumor cell line. Differential side scatter characteristics between effectors and targets were used to analyze conjugated and unconjugated cells. The previous labeling of NK and T cells with anti-Leu 11c and anti-Leu 4 monoclonal antibodies, allowed the distinction between unconjugated non-fluorescent and conjugated fluorescent targets and the percentual evaluation of bound anti-Leu 11c(+) and anti-Leu 4(+) cells.
建立了一种流式细胞术方法来评估外周血 NK 和 T 细胞与 K562 肿瘤细胞系的结合能力。使用效应细胞和靶细胞之间的差异侧向散射特性来分析缀合和未缀合的细胞。先前用抗 Leu11c 和抗 Leu4 单克隆抗体标记 NK 和 T 细胞，允许区分未缀合的非荧光和缀合的荧光靶细胞，并评估结合的抗 Leu11c(+)和抗 Leu4(+)细胞的百分比。
