Institute of Neurobiology, CNR, Viale Marx 15/43, 00137, Roma, Italy.
Cytotechnology. 1993 Jan;11(Suppl 1):S117-9. doi: 10.1007/BF00746073.
The neurite outgrowth and adhesion complex (NOAC), isolated from rabbit sera has been dissociated in its major components by reverse-phase chromatography in HPLC by using a C(18) column. SDS-PAGE analisys of the active fractions revealed the presence of three major bands of approximately 100, 70 and 50 kDa. Studies on the biological activity of NOAC were carried out on rat cerebellar granule cells. NOAC-cultured cells exhibit a marked resistance to excitotoxic stimuli carried by glutamate.
从兔血清中分离出的神经突生长和黏附复合物（NOAC），通过 HPLC 反相色谱在 C(18)柱上进行了主要成分的解离。活性部分的 SDS-PAGE 分析显示存在约 100、70 和 50 kDa 的三个主要条带。NOAC 的生物学活性研究是在大鼠小脑颗粒细胞上进行的。NOAC 培养的细胞对谷氨酸引起的兴奋毒性刺激表现出明显的抗性。
