Eboli M L, Ciotti M T, Mercanti D, Calissano P
Institute of General Pathology, Catholic University, Rome, Italy.
Neurochem Res. 1993 Feb;18(2):133-8. doi: 10.1007/BF01474675.
Cerebellar granule cells cultured in the presence of a differentiating factor isolated from rabbit serum exhibit, at variance with those cultured in fetal calf serum, an almost complete resistance to excitatory aminoacid (EAA)-induced cytotoxicity. We investigated the behaviour of protein kinase C (PKC), strongly implicated in EAA cytotoxicity, in the two types of culture. Phorbol esters, used to monitor the enzyme, enhanced the depolarization-evoked release of D-[3H]aspartate, but less effectively in factor-conditioned cells. EAAs increased phorbol esters binding in both cultures, but the effect was briefly lasting in factor-conditioned cells. The different behaviour of PKC is postulated to be causally related to different response to EAA of the cultures.
在存在从兔血清中分离出的分化因子的情况下培养的小脑颗粒细胞，与在胎牛血清中培养的细胞不同，对兴奋性氨基酸（EAA）诱导的细胞毒性几乎具有完全抗性。我们研究了在这两种培养物中，与EAA细胞毒性密切相关的蛋白激酶C（PKC）的行为。用于监测该酶的佛波酯增强了去极化诱发的D-[3H]天冬氨酸释放，但在因子处理的细胞中效果较差。EAA在两种培养物中均增加了佛波酯结合，但在因子处理的细胞中这种作用持续时间较短。PKC的不同行为被认为与培养物对EAA的不同反应存在因果关系。
