Cytotechnology. 1997 Sep;24(3):183-91. doi: 10.1023/A:1007931623160.
A recombinant Chinese hamster ovary (CHO) cell clone, S1, stably expressing human insulin-like growth factor binding protein-1 (hIGFBP-1), was treated with polyethylene glycol (PEG), resulting in cell fusion, in order to further enhance the protein expression by increasing the gene copy number and/or the amount of organelles important to the protein expression/-secretion. Both the fused cell line, Peg1, and its mother cell line, S1, were adapted to serum-free growth in suspension and were characterised with respect to growth and productivity. Peg1 was easier to adapt to the serum-free suspension conditions and had a higher viability during the adaptation period than S1. Furthermore, Peg1 showed a stable productivity of hIGFBP-1 that was twice as high as that for S1 under both adherent and suspension conditions. A considerable difference in the specific productivity (up to 3-4 times) was noticed during the growth phase. PEG fusion experiments have earlier been studied in our laboratory with CHO cells producing recombinant factor VIII and our results correlates very well with the results obtained with the factor VIII producing cells. Surprisingly, it was possible to obtain high producing recombinant cell lines, which were stable for more than 4 months.
一株稳定表达人胰岛素样生长因子结合蛋白-1（hIGFBP-1）的重组中国仓鼠卵巢（CHO）细胞克隆 S1 经聚乙二醇（PEG）处理后发生细胞融合，通过增加基因拷贝数和/或对蛋白质表达/分泌重要的细胞器的数量来进一步提高蛋白质表达水平。融合细胞系 Peg1 及其母系 S1 均适应于无血清悬浮培养，并对其生长和生产性能进行了表征。与 S1 相比，Peg1 更容易适应无血清悬浮条件，并且在适应期内具有更高的活力。此外，Peg1 在贴壁和悬浮条件下的 hIGFBP-1 生产能力均稳定，是 S1 的两倍。在生长阶段，比生产率（高达 3-4 倍）有明显差异。我们实验室之前已经对生产重组因子 VIII 的 CHO 细胞进行了 PEG 融合实验，我们的结果与生产因子 VIII 的细胞的结果非常吻合。令人惊讶的是，我们能够获得高产的重组细胞系，其稳定性超过 4 个月。
