用鼠源性 IgM 单克隆抗体 F(ab')2μ 片段通过流式细胞术分析人癌细胞上的唾液酸化路易斯 A 抗原。
Flow cytometric analysis of sialyl Lewis A antigen on human cancer cells by using F(ab')2μ fragments prepared from a mouse IgM monoclonal antibody.
出版信息
Cytotechnology. 1997 Sep;24(3):219-26. doi: 10.1023/A:1007940830409.
Abstract
F(ab')2 fragments, herein designated as F(ab')2μ fragments, were prepared from a mouse IgM monoclonal antibody specific to sialyl Lewis A antigen. The fragments were applied to flow cytometry to analyze the antigen on human cancer cells. The binding of the fragments to the antigen-positive cells was stronger than that of the original IgM. The non-specific binding of the IgM antibody to the antigen-negative cells was much decreased by using the F(ab')2μ fragments. These results indicate that the F(ab')2μ fragments are more suitable than the original IgM monoclonal antibody in flow cytometric analysis.
摘要
F(ab')2 片段，在此指定为 F(ab')2μ 片段，是从针对唾液酸化 Lewis A 抗原的鼠 IgM 单克隆抗体制备的。将片段应用于流式细胞术以分析人癌细胞上的抗原。片段与抗原阳性细胞的结合比原始 IgM 更强。使用 F(ab')2μ 片段后，IgM 抗体对抗原阴性细胞的非特异性结合大大减少。这些结果表明，在流式细胞术分析中，F(ab')2μ 片段比原始 IgM 单克隆抗体更合适。
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