Cangene Corporation, 104 Chancellor Matheson Road, R3T 5Y3, Winnipeg, Manitoba, Canada.
Cytotechnology. 1996 Jan;22(1-3):43-52. doi: 10.1007/BF00353923.
Two degradative activities were found in a recombinant Chinese hamster ovary cell culture. These activities became more dominant under high cell density and extended running time, as achieved in a semi-continous perfusion culture. The first, insulin degradative activity caused a growth upset in the 3rd cycle of the perfusion culture and shortened the length of the bioreactor process. The second activity, derived from the neutral pH stable sialidase, was found to affect the integrity of the carbohydrate structure of the recombinant protein, causing increase in heterogeneity in molecular weight and pI of the glycoforms. The most efficient way to overcome these problems may be the use of genetically altered 'designer cells' as the production cell line.
在重组中国仓鼠卵巢细胞培养物中发现了两种降解活性。这些活性在高细胞密度和延长运行时间下变得更加占优势，如在半连续灌注培养中实现的那样。第一种是胰岛素降解活性，它在灌注培养的第 3 个周期引起了生长失调，并缩短了生物反应器过程的长度。第二种活性来自于中性 pH 稳定的唾液酸酶，它被发现影响重组蛋白的碳水化合物结构的完整性，导致糖型的分子量和等电点异质性增加。克服这些问题的最有效方法可能是使用经过基因改造的“设计细胞”作为生产细胞系。
