Weidemann R, Ludwig A, Kretzmer G
Institut für Technische Chemie, Hannover FRG.
Cytotechnology. 1994;15(1-3):111-6. doi: 10.1007/BF00762385.
Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37 degrees C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
贴壁重组BHK细胞在30至37摄氏度之间的温度下培养。在2升生物反应器中进行的分批培养和重复分批培养对代谢和细胞生长有显著影响。低温培养显示生长速率较低，葡萄糖消耗率较低，因此乳酸产量也较低。另一方面，最大细胞密度和生产力似乎不受温度降低的影响。
