Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University Hung Hom, Kowloon, Hong Kong.
Cytotechnology. 1996 Jan;22(1-3):103-9. doi: 10.1007/BF00353929.
Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the protective effect is associated with the adsorption of the additives in the cell membrane through hydrophobic binding of the surface-active molecules to the membrane. Adsorption of the additives to the cell membrane may lead to decreased hydrophobicity of the cell surface, thus eliminating cell adhesion to bubbles and reducing cell damage from bursting bubbles. In this study, we measured the hydrophobicity of two insect cell lines based on cell adhesion to hydrocarbon phase and its influence by surface-active chemicals, Pluronic F68, a methylcellulose and a polyethylene glycol. The experimental results showed strong support for the aforecited cell protection mechanism.
悬浮培养的动物细胞可能会受到气泡破裂或其他水动力源产生的剧烈机械损伤。培养基中的某些化学添加剂，特别是一些表面活性剂，可以有效地保护动物细胞免受此类损伤。先前我们提出，这种保护作用与添加剂通过表面活性剂分子与细胞膜的疏水结合吸附在细胞膜上有关。添加剂吸附到细胞膜上可能会降低细胞表面的疏水性，从而消除细胞与气泡的附着，并减少气泡破裂对细胞的损伤。在这项研究中，我们基于细胞对烃相的附着及其受表面活性剂、Pluronic F68、甲基纤维素和聚乙二醇影响的情况，测量了两种昆虫细胞系的疏水性。实验结果强烈支持上述细胞保护机制。
