非离子表面活性剂对细菌表面疏水性的测定。
Determination of hydrophobicity on bacterial surfaces by nonionic surfactants.
作者信息
Noda Y, Kanemasa Y
出版信息
J Bacteriol. 1986 Sep;167(3):1016-9. doi: 10.1128/jb.167.3.1016-1019.1986.
Abstract
The hydrophobicity of the bacterial cell surface was determined by using nonionic surfactants. The method is based on the adsorption of nonionic surfactants at the hydrophobic sites of the cell surface. Among many nonionic surfactants, C18H37O(CH2CH2O)13H was preferred. The surfactant was added in excess to a bacterial suspension, and the suspension was mixed by sonication or mechanical stirring. The amount of surfactant remaining in the supernatant after centrifugation was determined spectrophotometrically by measuring the absorbance of tetrabromophenolphthalein ethylester. Effective dispersion of bacterial cells such as Staphylococcus aureus and Mycobacterium smegmatis was achieved by sonication in the presence of the nonionic surfactant. Adsorption measurements coincided with Langmuir's equation, indicative of monolayer adsorption. The method is useful for the determination of the hydrophobicity of various bacterial cell surfaces.
摘要
使用非离子表面活性剂测定细菌细胞表面的疏水性。该方法基于非离子表面活性剂在细胞表面疏水部位的吸附。在众多非离子表面活性剂中，优选C18H37O(CH2CH2O)13H。将过量的表面活性剂添加到细菌悬浮液中，通过超声处理或机械搅拌使悬浮液混合。离心后，通过测量四溴酚酞乙酯的吸光度，用分光光度法测定上清液中残留的表面活性剂含量。在非离子表面活性剂存在下，通过超声处理可有效分散金黄色葡萄球菌和耻垢分枝杆菌等细菌细胞。吸附测量结果符合朗缪尔方程，表明为单层吸附。该方法可用于测定各种细菌细胞表面的疏水性。
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